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CapitalBio Corporation
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Arrayit Corporation
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CapitalBio Corporation
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Digilab Inc
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Digilab Inc
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CapitalBio Corporation
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Amersham Pharmacia Biotech Ltd
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Labman Automation
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Molecular Dynamics Inc
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Amersham Pharmacia Biotech Ltd
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SCIENION
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Image Search Results
Journal: ACS chemical biology
Article Title: A Chemical Biology Solution to Problems with Studying Biologically Important but Unstable 9-O-Acetyl Sialic Acids
doi: 10.1021/acschembio.6b00928
Figure Lengend Snippet: Sialoglycan microarray binding specificity studies of human Siglec-9 (hSiglec-9-Fc, hSiglec-9), porcine torovirus hemagglutinin-esterase (PToV), and bovine coronavirus hemagglutinin-esterase (BCoV) (both PToV and BCoV were mutated to ablate their esterase activity) towards Neu5Ac9NAcα3Galβ4GlcβProNH2 (black columns), Neu5,9Ac2α3Galβ4GlcβProNH2 (gray columns), and Neu5Acα3Galβ4GlcβProNH2 (white columns) without esterase treatment (A) or treated with esterase active PToV (B).
Article Snippet: Glycan Microarray Screening Glycan microarrays were fabricated using epoxide-derivatized slides (Corning by Thermo Fisher Scientific) and
Techniques: Microarray, Binding Assay, Activity Assay
Journal: BMC Genomics
Article Title: Signal transduction-related responses to phytohormones and environmental challenges in sugarcane
doi: 10.1186/1471-2164-8-71
Figure Lengend Snippet: Validation of microarray results by quantitative PCR analysis. The y axis refers to the relative expression ratio between treated samples versus the control (untreated sample). (A) and (B) Real-time PCR results for ABA and MeJA treatments, respectively. The ratios were calculated in relation to the sample from untreated plants (0h). Transcript levels of the selected genes were profiled throughout the treatment time course. Also shown are the results for plant-endophytic bacteria association (C), drought (D), phosphate starvation (E) and herbivory (F). For these treatments, the real-time PCR reactions were carried out exclusively for the experimental point(s) in which the gene was considered differentially expressed. Only validated results are shown here. The RNA samples used in the real-time PCR experiments are from a third biological sample. All reactions were carried out in parallel and each reaction was performed in triplicates. Error bars were calculated as described previously [135]. Herb. = sample from plants inoculated with Herbaspirillum seropedicae and Herbaspirillum rubrisubalbicans ; Gluc. = sample from plants inoculated with Gluconacetobacter diazotrophicus . The transcript levels for the reference genes were verified to not vary in response to the treatments. The reference genes used encode a polyubiquitin gene (SCCCST2001G02.g [CA179923]) for the ABA and drought dataset, a GAPDH for the MeJA and herbivory dataset (retrieved from [38]), a 25S rRNA for the endophytic inoculation (retrieved from [38]) and a 14-3-3 gene (SCCCLR1048F12.g [CA119519]) for phosphate starvation
Article Snippet: Microarrays were constructed by arraying cDNA fragments on DMSO optimized metal-coated glass slides (type 7, GE Healthcare) using the
Techniques: Biomarker Discovery, Microarray, Real-time Polymerase Chain Reaction, Expressing, Control, Bacteria